Lipogems Method Increasingly Used for Orthopedic Conditions Professor Tremolada was interviewed by ClassHorse TV alongside Dr. Zeira, physician at San Michele Veterinary Hospital, to discuss Lipogems in veterinary applications. The treatment, which involves harvesting and grafting autologous adipose tissue, is increasingly utilized for orthopedic conditions not only in humans but also in domestic animals, horses, and beyond. Dr. Zeira has extensively employed Lipogems adipose tissue treatment to address conditions such as osteoarthritis, tendinopathies, ligament injuries, and joint problems in various animal species. The treatment, as in human medicine, involves adipose tissue harvesting similar to mini-liposuction, minimal manipulation of the aspirated fat, and inoculation into the target area. Liposuction and treatment occur in a single surgical procedure under aseptic conditions. Lipogems technology has demonstrated promising results in veterinary medicine due to its ability to preserve mesenchymal cells and the regenerative microenvironment of adipose tissue, promoting natural healing of damaged tissues.

Known for its potent antiviral and anti-inflammatory properties, oxygen-ozone therapy has been successfully used in regenerative medicine for various pathologies, particularly viral diseases like HIV and SARS, as well as in treating arthritis, ischemic heart disease, macular degeneration, cancer, wound disinfection, and immune system activation. Professor Carlo Tremolada, Scientific Director of Image Regenerative Clinic, explains. How does oxygen-ozone therapy work against coronavirus? According to recent studies shared by SIOOT, this procedure inhibits viral replication by balancing cellular redox state, activates and modulates the immune system, and improves oxygenation to prevent lung scarring and protect vital organs from viral damage. It is well documented that Italy, China, Spain, and the United States successfully employ oxygen-ozone therapy against SARS-CoV-2 infection. It is used both for hospitalized patients, including severe cases, as support to standard procedures, and for environmental disinfection. SIOOT also recommends oxygen-ozone therapy for prevention and post-illness recovery, as it strengthens the immune system and aids recovery. Why ozone? Used since World War I for wound disinfection and treating trench foot, ozone is a powerful anti-inflammatory and antiviral agent. Specifically regarding viral infections, it inhibits viral replication and provides direct virus inactivation. Its properties are numerous, leading to successful applications across medical fields including pulmonology, infectious diseases, dentistry, and dermatology. What does oxygen-ozone therapy involve? The therapy involves therapeutic and preventive use of an oxygen-ozone mixture, administered through various routes depending on the condition: autohemotherapy, intramuscular, intra-articular, subcutaneous, rectal, vaginal, uterine, or urethral administration, particularly for intestinal dysbiosis, candidiasis, and urinary tract infections. Once in the body, ozone releases oxygen atoms that attack viruses and bacteria. In aesthetic procedures, it is also administered through local applications with ozonized water, ozone-based creams, and ozonized oil. In which areas can oxygen-ozone therapy be used? Its anti-inflammatory properties make this procedure particularly effective for joint problems, such as meniscus deterioration and herniated discs, as well as vaginal atrophy and other menopausal disorders and recurrent candidiasis. In these cases, oxygen-ozone therapy can eliminate pain and irritation, sometimes proving definitive, especially when combined with Lipogems regenerative technology.

A Canadian study published in "JAMA Facial Plastic Surgery" by the American Medical Association reveals: botulinum toxin also rejuvenates the skin. The Clinical Trial The new potential of botulinum toxin was revealed through a study conducted on a sample of 43 women with an average age of 52 years who had never previously undergone Botox treatments. The women, who presented mild periocular wrinkles, received periodic injections of type A botulinum toxin, and their skin condition was evaluated over 4 months. Study Results The study results demonstrated significant improvement in skin quality and elasticity, measured through biomechanical instrumental analysis. The research showed that botulinum toxin stimulates skin biomechanics, promoting collagen production and reactivating the molecular skin rejuvenation process. This discovery, while opening new applications for botulinum toxin, confirms a concrete fact: when used correctly, botulinum toxin remains a drug with proven safety levels and significant therapeutic applications in aesthetic-functional medicine.

The NUTRINEXT® test analyzes Single Nucleotide Polymorphisms (SNPs) to provide the opportunity to develop personalized lifestyle strategies with our patients to prevent inflammation and subsequent damage to various tissues and organs in our body by eliminating risk factors. An advanced test (NGS sequencing), non-invasive (buccal swab), rapid and accurate, designed for individuals interested in personalized preventive medicine. The test analyzes how genes influence athletic performance, risk of sports injuries (bone fractures, joint problems), predisposition to Celiac disease, sensitivity to various substances (caffeine, lactose, fructose, alcohol, nickel, sulfites), cardiovascular health, weight management, and markers of oxidative stress and inflammatory response. The genetic information obtained enables the development of personalized preventive strategies and optimization of lifestyle choices based on individual genetic profiles. Molecular Medicine extends beyond ancestral analysis, becoming a fundamental tool for guiding lifestyle choices and preventing potential health issues encoded in our DNA. As Hippocrates stated in 371 BC, a healthy body is the foundation of an individual's overall well-being. Personalized preventive medicine enables us to implement this principle through advanced genomic analysis.

The tiger population has dramatically declined from 100,000 specimens in the early 1900s to fewer than 4,000 today, with precisely 3,890 individuals remaining in temperate and tropical regions worldwide. Lipogems Technology in Veterinary Medicine Lipogems, an innovative technology patented by Prof. Tremolada, harnesses the natural regenerative capabilities of adipose tissue. This procedure serves as either an alternative or complementary approach to surgical intervention, supporting tissue repair in both human and veterinary medicine. The treatment protocol, identical to human applications, involves autologous fat tissue harvesting through mini-liposuction, minimal tissue processing in a closed system, and injection into the target area. Both lipoaspiration and treatment occur in a single surgical procedure under aseptic conditions. Pioneering Cases: Tigers Treated with Lipogems Two significant cases have demonstrated Lipogems' effectiveness in exotic animal care. The first occurred in November 2016 when Dr. Zeira treated a Bengal tiger's cartilage issues at San Michele Veterinary Hospital. The second case in April 2018 gained international media attention, featured in Reuters, Washington Post, and New York Post, reaching audiences from Australia to Asia. Igor: The Siberian Tiger Case Study Igor, a 13-year-old Siberian tiger at Hungary's Szeged Zoo, received stem cell treatment for chronic hip joint pain. The procedure utilized Lipogems technology to harvest and process adipose tissue rich in mesenchymal stem cells. "The essence of stem cell treatment is that we can improve the quality of life for patients who cannot be helped by conventional operations or medical treatments," stated Robert Gippert, the veterinary surgeon who performed Igor's procedure. "Similar to human applications, Igor's worn joint will heal through the regenerative and self-repairing effects of stem cells, without requiring external materials," he added.

The results of the method patented by Prof. Tremolada will be presented at the conference taking place from October 5-7, 2018 in Croatia. The First Mediterranean Congress on Comprehensive Joint Care Distinguished guests from across Europe, the United Kingdom, and the United States will present their findings regarding new technologies for joint pain treatment. Among the invited speakers is Prof. Tremolada, who routinely performs conservative treatments with significant outcomes for various pathologies including degenerative joint arthrosis (shoulder, knee, ankle, hip) and beyond. The Lipogems technology enables the treatment of patients with articular cartilage lesions who often present with pain and reduced functionality. The procedure utilizes processed adipose tissue rich in mesenchymal stem cells and growth factors to promote tissue repair and regeneration. The conference will be held at Le Meridien Lav Hotel in Split, Croatia.

Lipogems takes center stage in rehabilitative research in regenerative medicine. An article on Eurekalert, a highly authoritative source for international scientific developments, reports on the research findings. The Kessler Foundation, a non-profit organization working to improve the lives of people with physical and cognitive disabilities (caused by stroke, multiple sclerosis, brain and spinal cord injuries, and other chronic neurological and musculoskeletal conditions) has received funding from the Derfner Foundation to support rehabilitation research in regenerative medicine and to train a scholarly doctoral group for careers in regenerative rehabilitation. This represents another significant recognition for Lipogems and Prof. Carlo Tremolada, who regularly uses micro-fragmented autologous adipose tissue injections to treat shoulder and rotator cuff pathologies. The Kessler Foundation's research is the first to examine the safety and efficacy of micro-fragmented adipose tissue injections for chronic shoulder pain, specifically in wheelchair users with spinal cord injuries. These individuals are highly susceptible to shoulder injuries, resulting in pain and reduced mobility. Shoulder surgery often fails to achieve desired outcomes, which is why global research is investigating viable non-surgical alternatives. The Lipogems treatment represents one of these promising alternatives. Rotator cuff injuries are a common cause of pain and loss of function among manual wheelchair users with SCI. When pharmacological treatment and physical therapy fail to address the pain, rotator cuff surgery is often the only option. Injection with micro-fragmented adipose tissue is being tested as an alternative when conservative treatments fail. The procedure involves harvesting, processing (using the Lipogems® system), and injection of a sample of the person's own fat into the shoulder joint under ultrasound guidance. Fat tissue provides cushioning and fills structural defects, and may deposit bioactive and regenerative elements in the damaged tissues. "So far, we've had six participants undergo the intervention and all have experienced better range of motion and less pain," noted Trevor Dyson-Hudson, MD, director of SCI Research at Kessler Foundation. "While results are preliminary, it appears that injection with adipose tissue prepared using the Lipogems system may be effective in treating shoulder pain from rotator cuff injuries in persons with SCI." The Kessler Foundation has recently joined the International Consortium for Regenerative Rehabilitation (ICRR), further strengthening its commitment to advancing regenerative medicine treatments. This collaboration will help facilitate the development and translation of new technologies that restore function and enhance quality of life for patients with disabilities.

Professor Tremolada's method takes center stage at the world congress on cartilage regeneration research Cartilage Repair and Reconstruction: Mesenchymal stem cells facilitate injury recovery, and Lipogems® represents the most efficient, rapid, and secure system for recovery and healing through autologous adipose tissue transplantation with natural site-specific deployment. ICRS 2016: Thirteenth World Congress Lipogems® will be one of the key topics discussed at the XIII World Congress of ICRS (International Cartilage Repair Society, an international scientific society directed by Japanese researcher Norimasa Nakamura) to be held in Sorrento from September 24-27, 2016. Professor Donato Rosa Discusses Lipogems® Professor Donato Rosa, Professor of Orthopedic Clinical Practice at the University of Naples 'Federico II', cites Lipogems® among the most significant innovations to be presented at the Sorrento congress. 'There are notable improvements in both diagnostic and therapeutic fields, but the most compelling aspect of this conference will be the discussion of emerging regenerative medicine techniques, including stem cell applications,' specifically mentioning 'Lipogems, which utilizes stem cells harvested from adipose tissue.' Referencing Professor Tremolada's patent, he concludes: 'These substances, combined with regenerative medicine surgical techniques, such as the filler already implemented in our Orthopedics and Traumatology Unit, enhance cartilage regeneration, facilitating rapid recovery and return to normal daily activities, recreation, and sports.' Image and Lipogems® Image The Urban Medi Spa, as the premier regenerative medicine clinic, takes pride in participating in international events of this caliber through Lipogems® and Professor Tremolada. The event will take place in Sorrento from September 24-27.

A clinical study conducted by Prof. Carlo Tremolada and Dr. G. A. Casarotti on urogenital atrophy treatment using the Lipogems method was presented at the AICPE National Congress in Florence. The study, initiated in 2015, involved 32 women who received a single Lipogems treatment, demonstrating significant efficacy in gynecological applications. A similar project is ongoing at Brown University's urogynecology department in Providence in collaboration with Istituto Image. Urogenital atrophy is a benign degenerative process affecting vaginal tissues and urethra, caused by depleted estrogen production. The condition typically progresses over time, leading to complete tissue atrophy. Vaginal Symptoms Common symptoms include vaginal dryness, dyspareunia, burning sensation, itching, and reduced clitoral and vaginal sensitivity. Increased collagen turnover reduces vaginal structural support, promoting vaginal prolapse. Reduced glycogen production leads to elevated vaginal pH, decreased lactobacillary flora, and increased coliform bacteria causing infections. Urethral Impact The urethra contains high levels of estrogen receptors due to its embryonic origin. Hypoestrogensim results in dysuria, bladder tenesmus, urinary burning, and stress urinary incontinence. Lipogems Treatment Outcomes The study monitored patients at 3, 6, 9, 12, 18, 24, and 36 months post-treatment. Initial findings showed significant symptom improvement by 6 months, with 25 patients reporting complete pain resolution at 12 months. Of 9 patients reaching the 36-month follow-up, only 2 reported mild residual symptoms. Laboratory analysis demonstrated restoration of vaginal pH from 6.0 ± 0.2 to 4.1 ± 0.2 and reestablishment of lactobacillary flora at 12-36 months post-treatment. Conclusion The Lipogems treatment demonstrated significant efficacy in addressing urogenital atrophy symptoms in postmenopausal women. Benefits included pain reduction, improved urinary symptoms, and restoration of normal vaginal flora and pH. The treatment's regenerative properties showed lasting effects through the 36-month follow-up period.

Dozens of articles are published annually in the USA discussing the remarkable results of Lipogems. The authors of these publications are surgeons who have often been personally trained by Prof. Tremolada in the use of Lipogems technology, which he invented and patented. A notable example is this article by physicians Jay Panchal, Gerard Malanga, and Mitchell Sheinkop, published in the American Journal of Orthopedics and featured in several authoritative online publications. Here is a brief excerpt: Key Study Concepts Knee osteoarthritis is a chronic condition affecting all races, genders, and ages, but predominantly presents in overweight and elderly individuals. Globally, arthritis is considered the fourth leading cause of disability. In both developing and developed countries, knee osteoarthritis can cause significant quality of life decline in individuals over 65 years due to pain and disability. Conservative, non-surgical treatments can be successful in patients with mild to moderate arthritic pain. Lipogems Mechanism of Action in Osteoarthritic Knees The FDA-approved Lipogems device is used to harvest adipose tissue, cleanse it of blood and oil components, and micro-fracture it while maintaining the intact vasculo-stromal niche containing pericytes. This study aimed to evaluate the safety and efficacy of minimally manipulated, micro-fractured autologous adipose tissue in patients with severe refractory knee osteoarthritis. Conclusions The injection of minimally manipulated, micro-fractured autologous adipose tissue represents a safe and effective treatment option for patients with severe refractory osteoarthritis (grade 3 or 4) of the knee. This study demonstrated significant improvements in pain, quality of life, and functionality for at least 12 months in the study cohort. Lipogems intervention may represent a non-surgical treatment option to avoid knee joint replacement in this population. Prof. Tremolada's Commentary "Lipogems is a method now used in over 20 countries with a growing success history of over 36,000 cases. To make its function comprehensible to everyone, I will simply say that it is currently the best technique used for adipose tissue transplantation."

This year again, Lipogems technology, patented by Prof. Tremolada, is featured at the world congress on joint preservation research, with particular focus on hip, foot, and knee conditions. VI Joint Preservation Congress - Warsaw For the third year, Prof. Tremolada returns to Warsaw to participate in the world congress. Most treatments used for degenerative diseases are unable to maintain full joint functionality and physical fitness in patients. With the increase in these conditions, increasingly affecting younger patients, professionals worldwide seek effective alternative treatments. The objective is to reduce patient pain and extend physical activity. The congress features leading international experts - prominent physicians and scientists including Prof. Carlo Tremolada - a leader in research, development, and application of new technologies for treating degenerative diseases and related trauma. Joint Preservation with Lipogems "I always aim to convey this new concept of regeneration. Lipogems is not stem cells, not nanofat, not a mechanically obtained SVF. Lipogems is a method for mechanically expanding the functional surface of adipose tissue through micro-fragmentation within a closed system. Lipogems not only improves vascular density but also initiates and significantly accelerates the natural healing process. With Lipogems, you heal like a child... You heal naturally." Lipogems represents the simplest, fastest, and safest system for injury recovery and healing. Patients with articular cartilage lesions experience pain and reduced functionality, often progressing to osteoarthritis, one of the top ten causes of disability worldwide. Lipogems dramatically accelerates the natural tissue regeneration and repair process. Through Lipogems, it's possible to conservatively treat various pathologies with exceptional results, including degenerative arthrosis affecting joints (shoulders, knees, ankles, hip) and more.

IV Joint Preservation Congress - Warsaw Current treatments for degenerative diseases are unable to maintain full joint functionality and patients' physical fitness. Due to the predicted continuous increase in these conditions, physicians worldwide are seeking effective alternative treatments. New therapies should aim to reduce patient pain and extend physical activity. The conference features world-renowned experts - physicians and scientists including Prof. Carlo Tremolada - who are leaders in developing and applying new technologies for treating degenerative diseases and related trauma. Joint Preservation with Lipogems Method Mesenchymal stem cells facilitate healing from trauma and injuries, and Lipogems® provides a simplified, rapid, and safe system for recovery by delivering these cells naturally through autologous adipose tissue transplantation. Patients with articular cartilage lesions often experience joint pain and reduced functionality, frequently progressing to osteoarthritis - one of the top 10 causes of disability worldwide. Lipogems accelerates the natural tissue regeneration and repair process, enabling conservative treatment of various pathologies including degenerative joint conditions affecting the shoulder, knee, ankle, and hip. The technology's unique processing method preserves the structural integrity of adipose tissue while maintaining its vascular network and extracellular matrix. This preservation ensures optimal integration and therapeutic efficacy when transplanted. The high concentration of mesenchymal stem cells present in processed adipose tissue, combined with growth factors and cytokines, promotes tissue repair and stimulates the body's natural healing response.