1. Home
  2. Scientific Literature
  3. Human adipose tissue micro-fragmentation for cell phenotyping and secretome characterization - Lipogems

Human adipose tissue micro-fragmentation for cell phenotyping and secretome characterization - Lipogems

B Vezzani, M Gomez

Abstract

In the past decade, adipose tissue transplants have been widely used in plastic surgery and orthopaedics to enhance tissue repletion and/or regeneration. Accordingly, techniques for harvesting and processing human adipose tissue have evolved in order to quickly and efficiently obtain large amounts of tissue. Among these, the closed system technology represents an innovative and easy-to-use system to harvest, process, and re-inject refined fat tissue in a short time and in the same intervention (intra-operatively). Adipose tissue is collected by liposuction, washed, emulsified, rinsed and minced mechanically into cell clusters of 0.3 to 0.8 mm. Autologous transplantation of mechanically fragmented adipose tissue has shown remarkable efficacy in different therapeutic indications such as aesthetic medicine and surgery, orthopedic and general surgery. Characterization of micro-fragmented adipose tissue revealed the presence of intact small vessels within the adipocyte clusters; hence, the perivascular niche is left unperturbed. These clusters are enriched in perivascular cells (i.e., mesenchymal stem cell (MSC) ancestors) and in vitro analysis showed an increased release of growth factors and cytokines involved in tissue repair and regeneration, compared to enzymatically derived MSCs. This suggests that the superior therapeutic potential of microfragmented adipose tissue is explained by a higher frequency of presumptive MSCs and enhanced secretory activity. Whether these added pericytes directly contribute to higher growth factor and cytokine production is not known. This clinically approved procedure allows the transplantation of …

Attention

The information available on the website istitutoimage.it is intended solely for informational purposes and is in no way a substitute for specialist medical advice. The contents are drafted in compliance with Art. 56 of the FNOMCeO Code of Ethics and the Ministerial Circular dated 18/12/2024. The information published has been prepared by qualified doctors and surgeons and is solely for educational purposes:

  • it does not in any way constitute a diagnosis;
  • it does not replace the direct doctor-patient relationship;
  • it must not be used as a prescription for treatments or as a basis for autonomous therapeutic decisions.

To assess suitability for aesthetic medicine treatments or plastic surgery procedures, a specialist medical consultation is always required.

For any questions, the Image Regenerative Clinic team is available with no obligation. Read the full Disclaimer.

Mission

This website is aimed at users seeking qualified and up-to-date information about regenerative aesthetic medicine, plastic surgery and longevity programs. Its contents are intended to provide general knowledge and are not a substitute for the doctor-patient relationship. Image Regenerative Clinic is committed to offering scientifically supported treatments and therapies with the utmost transparency and in full compliance with current regulations.

Website Authors

Dr. Carlo Tremolada – OMCEO Milan registration no. 30098  All the contents and images on this website have been written, reviewed and approved by the internal Medical-Scientific Committee coordinated by Dr. Carlo Tremolada, in compliance with Art. 56 of the FNOMCeO Code of Ethics and current regulations on healthcare advertising.

Website Ownership

The website istitutoimage.it is owned by Image Regenerative Clinic S.r.l.

It does not contain banner advertising, does not host sponsored content and does not benefit from grants or revenue from external advertising.