1. Home
  2. Comparison of Clinically Relevant Adipose Preparations on Articular Chondrocyte Phenotype in a Novel In Vitro Co-Culture Model - Lipogems

Comparison of Clinically Relevant Adipose Preparations on Articular Chondrocyte Phenotype in a Novel In Vitro Co-Culture Model - Lipogems

L. Kokai, J. Chen, D. Wang, S. Wang, F. M. Egro, B. Schilling, H. Sun, A. Ejaz, J. P. Rubin, J. A. Gusenoff, N. Vo, K. Onishi, G. Sowa

Abstract

Adipose therapeutics, including isolated cell fractions and tissue emulsifications, have been explored for osteoarthritis (OA) treatment; however, the optimal preparation method and bioactive tissue component for healing has yet to be determined. This in vitro study compared the effects of adipose preparations on cultured knee chondrocytes. De-identified human articular chondrocytes were co-cultured with adipose preparations for 36 or 72 h. Human adipose tissues were obtained from abdominal panniculectomy procedures and processed using three different techniques: enzymatic digestion to release stromal vascular fraction (SVF), emulsification with luer-to-luer transfer (nanofat), and processing in a bead-mill (Lipogems, Lipogems International SpA, Milan, Italy). Gene expression in both chondrocytes and adipose preparations was measured to assess cellular inflammation, catabolism, and anabolism. Results demonstrated that chondrocytes cultured with SVF consistently showed increased inflammatory and catabolic gene expression compared with control chondrocytes at both 36- and 72-h timepoints. Alternatively, chondrocytes co-cultured with either nanofat or bead-mill processed adipose derivatives yielded minimal pro-inflammatory effects and instead increased anabolism and regeneration of cartilage extracellular matrix. Interestingly, nanofat preparations induced transient matrix anabolism while Lipogems adipose consistently demonstrated increased matrix synthesis at both study timepoints after co-culture. This evaluation of the regenerative potential of adipose-derived preparations as a clinical tool for knee OA treatment suggests that mechanically processed preparations may be more efficacious than an isolated SVF cell preparation.

View Publication

Attention

The information available on the website istitutoimage.it is intended solely for informational purposes and is in no way a substitute for specialist medical advice. The contents are drafted in compliance with Art. 56 of the FNOMCeO Code of Ethics and the Ministerial Circular dated 18/12/2024. The information published has been prepared by qualified doctors and surgeons and is solely for educational purposes:

  • it does not in any way constitute a diagnosis;
  • it does not replace the direct doctor-patient relationship;
  • it must not be used as a prescription for treatments or as a basis for autonomous therapeutic decisions.

To assess suitability for aesthetic medicine treatments or plastic surgery procedures, a specialist medical consultation is always required.

For any questions, the Image Regenerative Clinic team is available with no obligation. Read the full Disclaimer.

Mission

This website is aimed at users seeking qualified and up-to-date information about regenerative aesthetic medicine, plastic surgery and longevity programs. Its contents are intended to provide general knowledge and are not a substitute for the doctor-patient relationship. Image Regenerative Clinic is committed to offering scientifically supported treatments and therapies with the utmost transparency and in full compliance with current regulations.

Website Authors

Dr. Carlo Tremolada – OMCEO Milan registration no. 30098  All the contents and images on this website have been written, reviewed and approved by the internal Medical-Scientific Committee coordinated by Dr. Carlo Tremolada, in compliance with Art. 56 of the FNOMCeO Code of Ethics and current regulations on healthcare advertising.

Website Ownership

The website istitutoimage.it is owned by Image Regenerative Clinic S.r.l.

It does not contain banner advertising, does not host sponsored content and does not benefit from grants or revenue from external advertising.